Toll-like receptors (TLRs) are now considered to be active players in tumor-host interactions. Toll-like receptor protein expression on/in cancer cell depends apparently on the level of RNA transcription. Based on previous work showing presence of TLR mRNA in six carcinoma cell lines, it was attempted to determine quantitatively mRNA of TLR-2, TLR-3 and TLR-4 by rt PCR. It has been shown that the expression of mRNA of three TLRs tested was uneven among examined cell lines. These data imply that in any future anti-tumor immunotherapeutic trials involving TLRs it seems mandatory to determine precisely TLR mRNA of cancer cells.