Experimental immunology
The influence of Rhodiola rosea extracts on non-specific and specific cellular immunity in pigs, rats and mice
The influence of Rhodiola rosea extracts on non-specific and specific cellular immunity in pigs, rats and mice
Publication date: 2007-07-31
Cent Eur J Immunol 2007;32(2):84-91
KEYWORDS
The genus Rhodiola (Crassulaceae) consists of more than 100 species growing mainly in Asia and Europe. The best known is Rhodiola rosea. The roots of this species are traditionally used as a tonicadaptogenantidepressant and anti-inflammatory drugs. The aim of our work was to study in vitro and in vivo the effects of aqueous and 50% hydroalcoholic extracts of Rhodiola rosea on non-specific and specific cellular immunity in pigsrats and mice. Mice were fed 50100200or 400 µg of Rhodiola extracts dailyfor 7 days before cellular immunity study (local GVH reaction). Blood leukocytes collected from pigs and rats were cultivated in vitro with PHA or LPS in the presence of 1-50 µg/ml of Rhodiola rosea extracts for 72 hoursThe metabolic activity of blood phagocytes (mostly granulocytes) was determined based on the measurement of intracellular respiratory burst after stimulation by PMA (phorbol myristate acetate)and potential bactericidal activity was determined in isolated blood leukocytes stimulated with microorganisms. Additionallysome in vitro toxicological studies were performed. For these experiments GMK (monkey’s kidney)EPC (fish epithelial cells)and KFC (Koy fins cells) cell linesas well as lymphocytes and monocytes isolated from the blood of pigs and rats were usedBoth extracts enhanced non-specific and specific cellular immunity to the various degreeshoweverin higher doses or concentrations they presented inhibitory effects. In in vitro studies all extracts werenon-toxic at concentrations 50400800and 1000 µg/ml after 2448and 72 hours of cells cultures
ABSTRACT
pigs, rats, mice, Rhodiola rosea, cellular immunity.
| eISSN: | 1644-4124 |
| ISSN: | 1426-3912 |
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