The amnion is considered as a rich source of factors beneficial for tissue regeneration. However, some studies revealed, that amniotic membrane also displays some anti-angiogenic properties.
The aim of the study was to compare the effect of radiation-sterilized and non-sterilized amnion dressings, on the proliferation of HECa10 endothelial cells (EC) in vitro. The influence of tissue samples on EC proliferation was evaluated with the Alamar Blue (AB) reagent.
Results: Non-sterilized amnion tissue significantly stimulated (p < 0.05) and radiation-sterilized samples highly significantly suppressed (p < 0.001) EC proliferation, as compare to the control cultures.
No difference was seen between the control fluorescence values (cultures with 10% of FBS) and cultures with non-sterilized amnion tissue homogenate. Significantly lower EC proliferation was observed in cell cultures containing homogenate of radiation-sterilized amnion membrane (p < 0.001).