The delayed wound healing is considered as important medical, social and economical problem. The effective wound management is one of major goals in health system strategy. Various dressing materials were used for this purpose among them skin and pericardium. The aim of the present paper was to compare the effect of these two materials, frozen and radiation-sterilized or non-sterilized, on the proliferation of HECa10 endothelial cells (EC) in vitro.
The influence of tissue samples on EC proliferation was evaluated with the Alamar Blue (AB) reagent, allowing the evaluation of viability and metabolic activity of cells.
Stimulation of EC proliferation was obtained for all used materials except sterilized pericardium, in comparison to the control cultures containing 10% of FBS (fetal bovine serum). No difference was observed between sterilized and non-sterilized skin.