The aim of this study was to assess occurrence of interleukin-1 receptor antagonist (IL-1Ra) and soluble tumor necrosis factor receptor (sTNF RI) in patients with chronic periodontitis, as well as to establish whether significant relationship exists between the cytokine inhibitors level and clinical parameters of periodontium. Significantly higher IL-1Ra concentration in saliva was observed in the chronic periodontitis group as compared with the control group. The correlation between IL-1Ra saliva concentration and plaque index, bleeding on probing and clinical attachment level has been found in the chronic periodontitis. The results suggest there is a link between saliva level of IL-1Ra and the ongoing inflammatory process in the periodontium.