Serological and virological survey on the occurrence of α-herpesvirus (BHV-1, BHV-5, BuHV-1, CpHV-1, CvHV-1, CvHV-2, ElkHV-1), pestivirus (BVDV-1, BVDV-2) and Coxiella burnetii infections in the population of wild ruminants in Poland was conducted. Sera from 40 randomly selected European bison from the Bialowieza Primeval Forest and 32 representatives of other wild ruminant species were used in the study. The sera were tested using commercial ELISA tests for antibodies against α -herpesviruses, pestiviruses and C. burnetii and Real Time PCR for pestivirus antigen. Only antibodies to pestiviruses were detected in the sera of two chital deer (Axis axis). No antibodies to α -herpesviruses and C. burnetii were found as well as the results of Real Time PCR were all negative. The results of the study imply that contact of Polish wild ruminants with these infections is very limited.