The aim of this study was to examine the effect of chromium and iron on interleukin-1α (IL-1α) and interleukin-6 (IL-6) concentration in vitro and in vivo.
The in vitro experiments were performed on the BALB/c 3T3 (mouse BALB/c embryo fibroblasts) cell line. The cells were incubated with 50 or 500 µM [Cr(H₂O)₄Cl₂]Cl × 2H₂O and 50 or 500 µM FeCl₃. Moreover, with 50 µM [Cr(H₂O)₄Cl₂]Cl × 2H₂O and 500 µM FeCl₃ or 50 µM FeCl₃ and 500 µm [Cr(H₂O)₄Cl₂]Cl × 2H₂O. The IL-1α and IL-6 concentration was determined. In vivo experiments were performed on NRMI mice, which were intraperitoneally injected with 0.5 ml: NaCl (control), 1 mg Cr, 10 mg Cr, 150 mg Fe, 300 mg Fe, 1 mg Cr and 150 mg Fe, 1 mg Cr and 300 mg Fe,10 mg Cr and 150 mg Fe,10 mg Cr and 300 mg Fe per body weight as chromium chloride and iron chloride solution.
Iron and chromium used separately increase statistically significant IL-1α concentration after incubation with 50 and 500 µM of iron chloride or chromium chloride, whereas they decrease statistically significant IL-6 concentration. Simultaneously, incubation BALB/c 3T3 cells with 50 µM chromium chloride and 500 µM iron chloride decreases statistically IL-1α concentration when compared with cells incubated with iron chloride at concentration of 500 µM. Simultaneously, incubation with 50 µM iron chloride and 500 µM chromium chloride increases statistically significant IL-1α concentration. Interleukin-6 concentration decreases when compared with control cells.
Simultaneously, an injection with iron and chromium in all combinations has increased statistically significant serum IL-1α and IL-6 concentration in all groups when compared with control animals.