Gram-positive rods of Lactobacillus rhamnosus GG (Lactobacillus GG) contribute to development and maturation of intestinal lymphatic system and, moreover, by increased IL-10 activity, decrease the expression of symptoms at the early stage of alimentary allergy and atopic dermatitis development. The aim of the study was to determine in vitro whether the metabolites of Lactobacillus rhamnosus GG stimulate the release of IL-10 from lymphocytes. The analyses were performed on whole human blood cell cultures using ELISA to determine the concentration of IL-10 in cultures liquid. Lactobacillus GG rods were immobilized in alginate capsules. To determine the tightness of the capsules their surfaces were observed by electron microscopy. During 14 days the migration of the bacteria to outside of the capsules was not observed. In cell cultures the increase in IL-10 release was determined, influenced by Lactobacillus GG metabolites contained in lyophilized alginate microcapsules, as compared to control group. The possible stimulants were exopolysaccharide and Lactobacillus GG metabolites, i.e. lactic acid, nitrogen oxide and hydrogen peroxide. Sodium alginate did not show synergistic activity with Lactobacillus GG metabolites.