Experimental immunology
Application of a flow cytometry in differentiation of antibodies accompanying platelets in Ehrlichia canis and Borrelia burgdorferi infections in dogs
Application of a flow cytometry in differentiation of antibodies accompanying platelets in Ehrlichia canis and Borrelia burgdorferi infections in dogs
Publication date: 2011-10-18
Cent Eur J Immunol 2011;36(3):174-179
KEYWORDS
ABSTRACT
Sera of dogs (n = 30), with serologically confirmed monocytic ehrlichiosis (Ehrlichia canis, n = 21) or borreliosis (Borrelia burgdorferi, n = 9) were tested for presence of antibodies accompanying platelets (anti-platelet antibodies – APA). The analysis was carried out in the flow cytometer based on the principle of indirect anti-globulin test, using FITC-labeled antiglobulin conjugate against canine IgG. Out of the 30 examined dogs, antibodies directed against thrombocytes were indicated in total 10 cases (33.3%).
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| eISSN: | 1644-4124 |
| ISSN: | 1426-3912 |
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