EXPERIMENTAL IMMUNOLOGY
The modulatory influence of some Echinacea-based remedies on antibody production and cellular immunity in mice
Submission date: 2015-11-29
Acceptance date: 2015-12-28
Publication date: 2016-03-24
Cent Eur J Immunol 2016;41(1):12-18
KEYWORDS
ABSTRACT
Echinacea purpurea-containing remedies are herbal medicines used in respiratory tract infections and several inflammatory conditions as enhancers of non-specific and modulators of specific cellular immunity. They also exert anti-inflammatory, anti-viral, and anti-microbial activity. The aim of the present study was to compare the in vivo influence of orally administered three Echinacea purpurea-based remedies (IMMUNAL drops, ECHINACEA FORTE drops, IMMUNAL FORTE tablets) on some parameters of cellular and humoral immunity in mice.
Results: Feeding mice for seven days with IMMUNAL drops resulted in enhanced anti-SRBC antibody production and modulatory effect on proliferative response to PHA of their splenic lymphocytes. No stimulatory effect was observed on splenocytes chemokinesis.
Mice fed with ECHINACEA FORTE drops presented enhanced response to PHA of their splenocytes. However, contrary to the previous group, no enhancement of antibody production was observed. In this group, lymphocyte-induced immunological angiogenesis (LIA) and chemokinesis (spontaneous migration – SM) of spleen lymphocytes was diminished after feeding mice with both doses (LIA) or with a higher dose (SM) of remedy. Lymphocyte-induced immunological angiogenesis activity of splenocytes collected from animals fed with prophylactic and therapeutic IMMUNAL FORTE tablet doses did not differ from the controls.
Results: Feeding mice for seven days with IMMUNAL drops resulted in enhanced anti-SRBC antibody production and modulatory effect on proliferative response to PHA of their splenic lymphocytes. No stimulatory effect was observed on splenocytes chemokinesis.
Mice fed with ECHINACEA FORTE drops presented enhanced response to PHA of their splenocytes. However, contrary to the previous group, no enhancement of antibody production was observed. In this group, lymphocyte-induced immunological angiogenesis (LIA) and chemokinesis (spontaneous migration – SM) of spleen lymphocytes was diminished after feeding mice with both doses (LIA) or with a higher dose (SM) of remedy. Lymphocyte-induced immunological angiogenesis activity of splenocytes collected from animals fed with prophylactic and therapeutic IMMUNAL FORTE tablet doses did not differ from the controls.
REFERENCES (33)
1.
Samochowiec E, Urbańska L, Mańka W, Stolarska E (1979): Assessment of the action of Calendula officinalis and Echinacea angustifolia extracts on Trichomonas vaginalis in vitro. Wiadomości Parazytologiczne 25: 77-81.
2.
Bany J, Zdanowska D, Zdanowski R, Skopińska-Różewska E (2003): The effect of herbal remedy on the development of Trichinella spiralis infection in mice. Pol J Vet Sci 6 (3 Suppl): 6-8.
3.
Egert D, Beuscher N (1992): Virus-inhibition by Echinacea purpurea. Planta Med 58: 163-165.
4.
Binns SE, Hudson J, Merali S, Arnason JT (2002): Antiviral activity of characterized extracts from Echinacea spp. (Heliantheae:Asteraceae) against herpes simplex virus (HSV-I). Planta Med 68: 780-783.
5.
Vimalanathan s, Kang L, Amiguet V, et al. (2005): Echinacea purpurea aerial parts contain multiple antiviral compounds. Pharmac Biol 43: 740-745.
6.
Sharma M, Schoop R, Suter A, Hudson JB (2011): The potential use of Echinacea in acne: control of Propionibacterium acnes growth and inflammation. Phytother Res 25: 517-521.
7.
Hudson JB (2012): Applications of the phytomedicine Echinacea purpurea (purple coneflower) in infectious diseases. J Biomed Biotechnol 2012: 769896.
8.
Bany J, Siwicki AK, Zdanowska D, et al. (2003): Echinacea purpurea stimulates cellular immunity and anti-bacterial defense independently of the strain of mice. Pol J Vet Sci 6 (suppl 3): 3-5.
9.
Bany J, Skopinska-Rozewska E, Joanna Chorostowska-Wynimko, et al. (2004): The effect of complex herbal remedy on the angiogenic activity of L-1 sarcoma cells, L-1 sarcoma tumor growth and on the bacterial infection in mice. Centr Eur J Immunol 29: 29-34.
10.
Hayashi I, Ohotsuki M, Suzuki I, et al. (2001): Effects of oral administration of Echinacea purpurea (American herb) on incidence of spontaneous leukemia caused by recombinant leukemia viruses in AKR/J mice. Nihon Rinsho Meneki Gakkai Kaishi 20: 10-20.
11.
Robbers JE, Tyler VE (1999): Herbs of choise: the therapeutic use of phytomedicinals. Haworth Herbal Press, New York.
12.
Molto J, Valle M, Miranda C, et al. (2011): Herb-drug interaction between Echinacea purpurea and darunavir-ritonavir in HIV- infected patients. Antimicrob Agents Chemother 55: 326-330.
13.
Mir-Rashed N, Cruz I, Jessulat M, et al. (2010): Disruption of fungal cell wall by antifungal Echinacea extracts. Med Mycol 48: 949-958.
14.
Chicca A, Adinolfi B, Martinotti E, et al. (2007): Cytotoxic effects of Echinacea root hexanic extracts on human cancer cell lines. J Ethnopharmacol 110: 148-153.
15.
Melchart D, Clemm C, Weber B, et al. (2002): Polysaccharides isolated from Echinacea purpurea herba cell cultures to counteract undesired effects of chemotherapy – a pilot study. Phytother Res 16: 138-142.
16.
Yu D, Yuan Y, Jiang L, et al. (2013): Anti-inflammatory effects of essential oil in Echinacea purpurea L. Pak J Pharm Sci 26: 403-408.
17.
Skopińska-Różewska E, Sokolnicka I, Siwicki AK, et al. (2011): Dose-dependent in vivo effect of Rhodiola and Echinacea on the mitogen-induced lymphocyte proliferation in mice. Pol J Vet Sci 14: 265-272.
18.
Sandberg G (1976): The sealed capillary migration technique and thymocyte migration in vitro. J Immunol Meth 12: 365-368.
19.
Skopińska-Różewska E, Bychawska M, Białas-Chromiec B, Sommer E (2009): The in vivo effect of Rhodiola rosea and Rhodiola quadrifida hydro-alcoholic extracts on chemokinetic activity of spleen lymphocytes in mice. Cent Eur J Immunol 34: 42-45.
20.
Skopińska-Różewska E, Wasiutyński A, Sommer E, et al. (2011) : Modulatory effect of Echinacea pallida on cellular immunity and angiogenesis in mice. Cent Eur J Immunol 36: 18-23.
21.
Skopiński P, Lewicki S, Bałan BJ, et al. (2014): In vivo inhibitory effect of Aloe vera gel on the ability of mouse parental splenic lymphocytes to induce cutaneous angiogenesis in recipient F1 mice. Pol J Vet Sci 17: 131-136.
22.
Bałan BJ, Nartowska J, Skopińska-Różewska E, et al. (2007): Wpływ wyciągów Echinacea purpurea na reakcje odpornościowe oraz procesy angiogenezy. In: Endogenne i egzogenne modulatory odporności i angiogenezy. Skopińska-Różewska E, Siwicki AK (eds.). Wyd. EDYCJA, Olsztyn; pp. 27-60.
23.
Sokolnicka I, Skopińska-Różewska E, Strzelecka H, et al. (2001): Adaptacja testów biologicznych do oceny aktywności preparatów jeżówki purpurowej (Echinacea purpurea). 1. Badania in vivo. Terapia 3: 38-42.
24.
Skopińska-Różewska E, Furmanowa M, Guzewska J, et al. (2002): The effect of Centella asiatica,Echinacea purpurea and Melaleuca alternifolia on cellular immunity in mice. Cent Eur J Immunol 27: 142-148.
25.
Woelkart K, Bauer R (2007): The role of alkamides as an active principle of echinacea. Planta Medica 73: 615-623.
26.
Chicca A, Raduner S, Pellati F, et al. (2009): Synergistic immunopharmacological effects of N-alkylamides in Echinacea purpurea herbal extracts. Int Immunopharmacol 9 (7-8): 850-858.
27.
Perry NB, van Klink JW, Burgess EJ, Parmenter GA (2000): Alkamide levels in Echinacea purpurea: effects of processing, drying and storage. Planta Med 66: 54-56.
28.
Kim HO, Durance TD, Scaman CH, Kitts DD (2000): Retention of alkamides in dried Echinacea purpurea. J Agric Food Chem 48: 4187-4192.
29.
Livesey J, Awang DV, Amason JT, et al. (1999): Effect of temperature on stability of marker constituents in Echinacea purpurea root formulations. Phytomedicine 6: 347-349.
30.
Stuart DL, Wills RB (2003): Effect of drying temperature on alkylamide and cichoric acid concentrations of Echinacea purpurea. J Agric Food Chem 51: 1608-1610.
31.
Matthias A, Addison RS, Agnew LL, et al. (2007): Comparison of Echinacea alkylamide pharmacokinetics between liquid and tablet preparations. Phytomedicine 14: 587-590.
32.
Glinkowska G, Bałan B, Sommer E, et al. (1997): The effect of phenolic compounds of poplar leaves extract on cutaneous angiogenesis reaction induced in mice by human mononuclear leukocytes. Acta Pol Pharm 54: 151-154.
33.
Bałan BJ, Skopińska-Różewska E, Barcz E, et al. (1999): Wpływ wybranych kwasów fenolowych na aktywność angiogenną komórek raka jajnika – doniesienie wstępne. Onkol Pol 2: 203-208.
Share
RELATED ARTICLE
| eISSN: | 1644-4124 |
| ISSN: | 1426-3912 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
