EXPERIMENTAL IMMUNOLOGY
The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells
Submission date: 2016-11-09
Acceptance date: 2016-11-14
Publication date: 2017-12-30
Cent Eur J Immunol 2017;42(4):331-335
KEYWORDS
ABSTRACT
In previous work we described the decline in the number of splenocytes of mice which during pregnancy and lactation were fed Rhodiola kirilowii. In this work we present the size of individual subpopulations of splenic lymphocytes in these mice. Experiments were performed on adult inbred female Balb/c mice, 8-9 weeks old, 20-22 γ b.m., mated with adult males from the same strain. Females, from when the copulatory plug was noted up to the 28th day after delivery, were supplemented daily with lyophilized aqueous (RKW) or 50% hydro-ethanolic (RKW-A) extract (20 mg/kg b.m.) dissolved in distilled water. Then, mice were euthanized, spleens dissected, cells counted and the total numbers of CD3+, CD19+, CD4+, CD8+ and CD335+ splenic lymphocytes were evaluated by cytometry.
The number of CD3+ lymphocytes per 1 γ of splenic tissue was higher in RKW-A than in RKW spleens and did not differ from the control. The number of CD3+ lymphocytes in RKW spleens was lower than in the controls. The number of CD19+ and CD4+ cells was lower in both experimental groups than in the controls. The number of CD335+(NK) cells was lower in RKW spleens than in the control.
The number of CD3+ lymphocytes per 1 γ of splenic tissue was higher in RKW-A than in RKW spleens and did not differ from the control. The number of CD3+ lymphocytes in RKW spleens was lower than in the controls. The number of CD19+ and CD4+ cells was lower in both experimental groups than in the controls. The number of CD335+(NK) cells was lower in RKW spleens than in the control.
REFERENCES (27)
1.
Zhang JQ, Meng SY, Rao GY (2014): Phylogeography of Rhodiola kirilowii (Crassulaceae): a story of Miocene divergence and quaternary expansion. PLoS One 9: e112923.
2.
Chen L, Yu B, Zhang Y, et al. (2015): Bioactivity-guided fractionation of an antidiarrheal Chinese herb Rhodiola kirilowii (Regel) Maxim reveals (–)-epicatechin-3-gallate and (–)-epigallocatechin-3-gallate as inhibitors of cystic fibrosis transmembrane conductance regulator. PLoS One 10: e0119122.
3.
Grech-Baran M, Sykłowska-Baranek K, Pietrosiuk A (2015): Approaches of Rhodiola kirilowii and Rhodiola rosea field cultivation in Poland and their potential health benefits. Ann Agric Environ Med 22: 281-285.
4.
Wójcik R, Siwicki AK, Skopińska-Różewska E, et al. (2009): The effect of Chinese medicinal herb Rhodiola kirilowii extracts on cellular immunity in mice and rats. Pol J Vet Sci 12: 399-405.
5.
Gao XF, Shi HM, Sun T, Ao H (2009): Effects of Radix et rhizome Rhodiolae kirilowii on expressions of Willebrand factor, hypoxia- inducible factor 1 and VEGF in myocardium of rats with acute myocardial infarction. Zhong Xi Jie He Xue Bao 7: 434-440.
6.
Skopińska-Różewska E, Bychawska M, Białas-Chromiec B, et al. (2010): The in vivo effect of Rhodiola kirilowii extracts on blood granulocytes metabolic activity in mice. Cent Eur J Immunol 35: 20-24.
7.
Siwicki AK, Skopinska-Różewska E, Wasiutyński A, et al. (2012): The effect of Rhodiola kirilowii extracts on pigs’ blood leukocytes metabolic (RBA) and proliferative (LPS) activity, and on the bacterial infection and blood leukocytes number in mice. Cent Eur J Immunol 37: 145-150.
8.
Zuo G, Li Z, Chen L, Xu X (2007): Activity of compounds from Chinese herbal medicine Rhodiola kirilowii (Regel)Maxim against HCV NS3 serine protease. Antiviral Res 76: 86-92.
9.
Wong YC, Zhao M, Zong YY, et al. (2008): Chemical constituents and anti-tuberculosis activity of root of Rhodiola kirilowii. Zhongguo Zhong Yao Za Zhi 33: 1561-1565.
10.
Lewicki S, Stankiewicz W, Skopinska-Różewska E, et al. (2015): Spleen content of selected polyphenols, splenocytes morphology and function in mice fed Rhodiola kirilowii extracts during pregnancy and lactation. Pol J Vet Sci 18: 847-855.
11.
Zdanowski R, Lewicki S, Sikorska K, et al. (2014): The influence of aqueous and hydro-alcoholic extracts of roots and rhizomes of Rhodiola kirilowii on the course of pregnancy in mice. Cent Eur J Immunol 39: 471-475.
12.
Singh BN, Shankar S, Srivastava RK (2011): Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol 82: 1807-1821.
13.
Yoshida N, Kuriyama I, Yoshida H, Mizushina Y (2013): Inhibitory effects of catechin derivatives on mammalian DNA polymerase and topoisomerase activities and mouse one-cell zygote development. J Biosci Bioeng 115: 303-309.
14.
Hushmendy S, Jayakumar L, Hahn AB, et al. (2009): Select phytochemicals suppress human T-lymphocytes and mouse splenocytes suggesting their use in autoimmunity and transplantation. Nutr Res 29: 568-578.
15.
Pae M, Ren Z, Meydani M, et al. (2010): Epigallocatechin- 3-gallate directly suppresses T cell proliferation through impaired IL-2 utilization and cell cycle progression. J Nutr 140: 1509-1515.
16.
Correia M, Sousa MI, Rodrigues AS, et al. (2016): Data on the potential impact of food supplements on the growth of mouse embryonic stem cells. Data Brief 7: 1190-1195.
17.
Li H, Yang L, Zhang Y, Gao Z (2016): Kaempferol inhibits fibroblast collagen synthesis, proliferation and activation in hypertrophic scar via targeting TGF- receptor type I. Biomed Pharmacother 83: 967-974.
18.
Correia M, Rodrigues AS, Perestrelo T, et al. (2015): Different concentrations of kaempferol distinctly modulate murine embryonic stem cell function. Food Chem Toxicol 87: 148-156.
19.
Mutlu Altundagˇ E, KasacI T, YIlmaz A, et al. (2016): Quercetin-Induced Cell Death in Human Papillary Thyroid Cancer (B-CPAP) Cells. J Thyroid Res 2016: 9843675.
20.
van der Woude H, Gliszczyńska-Swigło A, Struijs K, et al. (2003): Biphasic modulation of cell proliferation by quercetin at concentrations physiologically relevant in humans. Cancer Lett 200: 41-47.
21.
Kang S, Wang J (1997): Comparative study of the constituents from 10 Rhodiola plants. Zhong Yao Cai 20: 616-618.
22.
Gryszczyńska A, Łowicki Z, Opala B, et al. (2013): Determination of lotaustralin in Rhodiola species. Herba Polonica 59; DOI: 10.2478/hepo-2013-0008.
23.
Peng JN, Ma CY, Ge YC (1994): Chemical constituents of Rhodiola kirilowii (Regel). Zhongguo Zhong Yao Za Zhi 19: 676-702.
24.
Miyagawa F, Okiyama N, Villarroel V, Katz SI (2013): Identification of CD3+CD4-CD8- T cells as potential regulatory cells in an experimental murine model of graft-versus-host skin disease (GVHD). J Invest Dermatol 133: 2538-2545.
25.
Getachew Y, Cusimano FA, James LP, Thiele DL (2014): The role of intrahepatic CD3+/CD4/CD8 double negative T (DN T) cells in enhanced acetaminophen toxicity. Toxicol Appl Pharmacol 280: 264-271.
26.
Grishkan IV, Ntranos A, Calabresi PA, Gocke AR (2013): Helper T cells down-regulate CD4 expression upon chronic stimulation giving rise to double-negative T cells. Cell Immunol 284: 68-74.
27.
Li Y, Yao J, Han C, et al. (2016): Quercetin, Inflammation and Immunity. Nutrients 8: 167.
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| eISSN: | 1644-4124 |
| ISSN: | 1426-3912 |
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