EXPERIMENTAL IMMUNOLOGY
Structure and antigenicity analysis of the IgG gene for Nyctereutes procyonoides
Submission date: 2015-03-13
Final revision date: 2015-04-07
Acceptance date: 2015-05-04
Publication date: 2015-10-15
Cent Eur J Immunol 2015;40(3):271-275
KEYWORDS
ABSTRACT
Objective: Nyctereutes procyonoides immunoglobulin G (IgG) gene is partially cloned.
Material and methods: In order to obtain a certain length (966bp) of Nyctereutes procyonoides immunoglobulin G (IgG), two pairs of primers are designed according to the conserved nucleotide sequence of canine (GenBank:AF354265, AF354265, AF354266, AF354267) and mink (GenBank: L07789). Using Bioinformatics technology and Western-blot to analyze antigenicity of Nyctereutes procyonoides IgG-B gene.
Results: The homology for nucleotide sequence of IgG between Nyctereutes procyonoides and canine (IgG A, IgG B, IgG C, IgG D), mink, Homo sapiens, Oryctolagus cuniculus, Mus musculus, Anas platyrhynchos and gallus were respectively (88.1%, 93.6%, 85.4%, 87.2%), 83.7%, 74.8%, 71.8%, 69.2%, 51.6%, 48.4%. It can be seen that there was high homology of aminoacid sequence between IgG of Nyctereutes procyonoides and IgG (A, B, C, D) of canine. And the serum antibody of Nyctereutes procyonoides had obviously cross-reaction with HRP conjugated rabbit anti-dog IgG,compared with those of canine, oryctolagus cuniculus, mus musculus, mink, gallus.
Conclusions: We successfully got Nyctereutes procyonoides immuneglobulin G (IgG) gene (GenBank: KM010191). There is the closest ties of consanguinity of IgG exist between Nyctereutes procyonoides and canine among the mammal through the genetic evolution.The detection and treament of canine distemper can be used on Nyctereutes procyonoides.
Material and methods: In order to obtain a certain length (966bp) of Nyctereutes procyonoides immunoglobulin G (IgG), two pairs of primers are designed according to the conserved nucleotide sequence of canine (GenBank:AF354265, AF354265, AF354266, AF354267) and mink (GenBank: L07789). Using Bioinformatics technology and Western-blot to analyze antigenicity of Nyctereutes procyonoides IgG-B gene.
Results: The homology for nucleotide sequence of IgG between Nyctereutes procyonoides and canine (IgG A, IgG B, IgG C, IgG D), mink, Homo sapiens, Oryctolagus cuniculus, Mus musculus, Anas platyrhynchos and gallus were respectively (88.1%, 93.6%, 85.4%, 87.2%), 83.7%, 74.8%, 71.8%, 69.2%, 51.6%, 48.4%. It can be seen that there was high homology of aminoacid sequence between IgG of Nyctereutes procyonoides and IgG (A, B, C, D) of canine. And the serum antibody of Nyctereutes procyonoides had obviously cross-reaction with HRP conjugated rabbit anti-dog IgG,compared with those of canine, oryctolagus cuniculus, mus musculus, mink, gallus.
Conclusions: We successfully got Nyctereutes procyonoides immuneglobulin G (IgG) gene (GenBank: KM010191). There is the closest ties of consanguinity of IgG exist between Nyctereutes procyonoides and canine among the mammal through the genetic evolution.The detection and treament of canine distemper can be used on Nyctereutes procyonoides.
REFERENCES (15)
1.
Rikula U, Pänkälä L, Jalkanen L, et al. (2001): Distemper vaccination of farmed fur animals in Finland. Prev Vet Med 49: 125-133.
2.
Martella V, Cirone F, Elia G, et al. (2006): Heterogeneity within the hemagglutinin genes of canine distemper virus (CDV) strains detected in Italy. Vet Microbiol 116: 301-309.
3.
Navolotskaya EV (2014): The second life of antibodies. Biochemistry (Moscow) 79: 1-7.
4.
Jefferis R, Lund J, Pound JD. IgG-Fc-mediated effector functions: molecular definition of interaction sites for effector ligands and the role of glycosylation. Immunol Rev 1998; 163: 59-76.
5.
Morgan EL, Thoman ML, Weigle WO (1981): Enhancement of T lymphocyte functions by Fc fragments of immunoglobulins. I. Augmentation of allogeneic mixed lymphocyte culture reactions requires I-A-or I-B-subregion differences between effector and stimulator cell populations. J Exp Med 153: 1161-1172.
6.
Krapp S, Mimura Y, Jefferis R, et al. (2003): Structural analysis of human IgG-Fc glycoforms reveals a correlation between glycosylation and structuralintegrity. J Mol Biol 3255: 978-989.
7.
Taylor AI, Sutton BJ, Calvert RA (2010): Mutations in an avian IgY-Fc fragment reveal the locations of monocyte Fc receptor binding sites. Dev Comp Immunol 34: 97-101.
8.
Cherpillod P, Beck K, Zurbriggen A, Wittek R (1999): Sequence analysis and expression of the attachment and fusion proteins of canine distemper virus wild-type strain A75/17. J Virol 73: 2263-2269.
9.
Keawcharoen J, Theamboonlers A, Jantaradsamee P, et al. (2005): Nucleotide sequence analysis of nucleocapsid protein gene of canine distemper virus isolates in Thailand. Vet Microbiol 105: 137-142.
10.
Yoshida E, Shin YS, Iwatsuki K, et al. (1999): Epitopes and nuclear localization analyses of canine distemper virus nucleocapsid protein by expression of its deletion mutants. Vet Microbiol 66: 313-320.
11.
Plattet P, Cherpillod P, Wiener D, et al. (2007): Signal peptide and helical bundle domains of virulent canine distemper virus fusion protein restrict fusogenicity. J Virol 81: 11413-11425.
12.
Tsurudome M, Ito M, Nishio M, et al. (2011): Identification of domains on the fusion (F) protein trimer that influence the hemagglutinin-neuraminidasespecificity of the f protein in mediating cell-cell fusion. J Virol 85: 3153-2161.
13.
Sheshberadaran H, Norrby E, McCullough KC, et al. (1986): The antigenic relationship between measles, canine distemper and rinderpest viruses studied with monoclonal antibodies.J Gen Virol 1986; 67 (Pt 7): 1381-1392.
14.
Tatsuo H, Ono N, Yanagi Y (2001): Morbilliviruses use signaling lymphocyte activation molecules (CD150) as cellular receptors. J Virol 75: 5842-5850.
15.
Zhao J, Zhang H, Gao H, et al. (2010): Fox, raccoon and mink distemper virus cloning and eukaryotic expression of the receptor SLAM. Mammals Journal 2010; 30: 79-86.
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