The aim of presented studies was to determine the effect of total pea albumins on mice immune system response. Oral and intraperitoneal (i.p.) route of antigen administration were checked and three different adjuvants (Freund adjuvant, alum and cholera toxin) were used. All three groups showed enhancement of serum IgG and IgA antibodies production. Using cholera toxin (CT) to oral immunization with pea albumins gave comparable antibodies level to intraperitoneal immunizations, and the best T cell response.
Group orally immunized with pea albumins (PA) with CT as adjuvant resulted in high percentage of CD4 T cells population. We conluded, that the oral model of Balb/c mice immunization with antigen and CT as adjuvant, could be used to experiments determining food proteins immunogenicity and response of effector tissue of mucosal immune system.