Prolactin itself exerts multiple immunomodulatory effects and prolactin circulating level changes in the course of some immune-inflammatory diseases. Since little has been known about the relationship between the neuroimmunoendocrine system and atopic allergy, the present study focused on the behaviour of basal prolactin concentration in the peripheral blood of patients with atopic eczema/dermatitis syndrome (AEDS). Serum concentration of prolactin was measured by the electrochemiluminescence immunoassay (ECLIA) method in 13 female patients with severe intensity of AEDS and compared next with 14 healthy subjects. Blood was taken at 8 a.m. for measurement of basal prolactin concentration. Prolactin concentration values did not differ significantly among the two groups. It seems that increased secretion of prolactin does not accompany the immune-inflammatory processes associated with AEDS.