In our previous study we presented age-dependence of human serum angiogenic activity, evaluated in vivo after injection into mouse skin. We have found negative correlation between the age of serum donor and in vivo activity of serum, negative correlation between age and VEGF serum concentration and positive correlation between age and IL-18 level. The aim of the present study was to evaluate in vivo angiogenic activity as well as concentration of VEGF and antithrombin-III in sera collected from 30 healthy women and 30 healthy men of various age (20 – 80 years old), and to analyze whether some relations exist between studied parameters and gender. In men, we have found : negative correlation between the age and serum in vivo activity, negative correlation between the age and serum VEGF concentration, positive correlation between the age and serum AT-III activity. In women, no such relations were observed.