EXPERIMENTAL IMMUNOLOGY
MicroRNA-20b promotes the accumulation of CD11b+Ly6G+Ly6Clow myeloid-derived suppressor cells in asthmatic mice
Submission date: 2016-01-05
Final revision date: 2016-02-19
Acceptance date: 2016-02-24
Publication date: 2017-05-08
Cent Eur J Immunol 2017;42(1):30-38
KEYWORDS
ABSTRACT
miR-20b is a member of the miR-106a-363 gene cluster, which has been shown to play an important role in a variety of diseases, including cancer, inflammation, and autoimmune diseases. Our previous study indicated that miR-20b has an inhibitory effect on airway inflammation in asthmatic mice, but the exact mechanism is unclear. In this study, we report that the ratio of CD11b+Ly6G+Ly6Clow cells, but not the amount of CD11b+Ly6C+Ly6G– cells, was increased in the lung tissue of asthmatic mice after intranasal instillation with miR-20b mimics, while Th2-type cytokines (interleukin (IL)-4 and IL-13) were significantly decreased in the bronchoalveolar lavage fluid. In addition, the transcription factor CREB regulated the expression of miR-20b. Our findings suggest that miR-20b can induce the accumulation of myeloid-derived suppressor cells in the lungs of asthmatic mice, which may be a mechanism by which miR-20b inhibits airway inflammation in asthmatic mice. Thus, miR-20b may be used as a target for the effective treatment of asthma in the future.
REFERENCES (31)
1.
Pelaia G, Vatrella A, Busceti MT, et al. (2015): Cellular mechanisms underlying eosinophilic and neutrophilic airway inflammation in asthma. Mediators Inflamm 2015: 879783.
2.
Fahy JV (2015): Type 2 inflammation in asthma – present in most, absent in many. Nat Rev Immunol 15: 57-65.
3.
Olin JT, Wechsler ME (2014): Asthma: pathogenesis and novel drugs for treatment. BMJ 349: g5517.
4.
Su Z, Yang Z, Xu Y, et al. (2015): MicroRNAs in apoptosis, autophagy and necroptosis. Oncotarget 11: 8474-8490.
5.
Perry MM, Adcock IM, Chung KF (2015): Role of microRNAs in allergic asthma: present and future. Curr Opin Allergy Clin Immunol 2: 156-162.
6.
Liu F, Qin HB, Xu B, et al. (2015): Profiling of miRNAs in pediatric asthma: upregulation of miRNA-221 and miRNA-485-3p. Mol Med Rep 5:1178-1182.
7.
Collison A, Herbert C, Siegle JS, et al. (2011): Altered expression of microRNA in the airway wall in chronic asthma: miR-126 as a potential therapeutic target. BMC Pulm Med 11: 29.
8.
Panganiban RP, Pinkerton MH, Maru SY, et al. (2012): Differential microRNA epression in asthma and the role of miR-1248 in regulation of IL-5. Am J Clin Exp Immunol 2: 154-165.
9.
Tsitsiou E, Williams AE, Moschos SA, et al. (2012): Transcriptome analysis shows activation of circulating CD8+ T cells in patients with severe asthma. J Allergy Clin Immunol 129: 95-103.
10.
Song C, Ma H, Yao C, et al. (2012): Alveolar macrophage-derived vascular endothelial growth factor contributes to allergic airway inflammation in a mouse asthma model. Scand J Immunol 75: 599-605.
11.
Ma H, Luo YL, Guo SJ, et al. (2015): Inhibitory effect of miR-20b on airway inflammation in asthmatic mice. Nan Fang Yi Ke Da Xue Xue Bao 35: 1463-1466.
12.
Song C, Yuan Y, Wang XM, et al. (2014): Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation. Scand J Immunol 79: 98-104.
13.
Nagaraj S, Youn JI, Gabrilovich DI (2013): Reciprocal relationship between myeloid-derived suppressor cells and T cells. J Immunol 191: 17-23.
14.
Lindau D, Gielen P, Kroesen M, et al. (2013): The immunosuppressive tumour network: myeloid-derived suppressor cells, regulatory T cells and natural killer T cells. Immunology 138: 105-115.
15.
Gabrilovich DI, Nagaraj S (2009): Myeloid-derived suppressor cells as regulators of the immune system. Nat Rev Immunol 9: 162-174.
16.
Jiang J, Guo W, Liang X (2014): Phenotypes, accumulation, and functions of myeloid-derived suppressor cells and associated treatment strategies in cancer patients. Hum Immunol 75: 1128-1137.
17.
Chen S, Zhang Y, Kuzel TM, et al. (2015): Regulating Tumor Myeloid-Derived Suppressor Cells by MicroRNAs. Cancer Cell Microenviron 2 pii: e637.
18.
Liu Y, Lai L, Chen Q, et al. (2012): MicroRNA-494 is required for the accumulation and functions of tumor-expanded myeloid-derived suppressorcells via targeting of PTEN. J Immunol 188: 5500-5510.
19.
Li L, Zhang J, Diao W, et al. (2014): MicroRNA-155 and MicroRNA-21 promote the expansion of functional myeloid-derived suppressor cells. J Immunol 192: 1034-1043.
20.
Mei S, Xin J, Liu Y, et al. (2015): MicroRNA-200c Promotes Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating PTEN andFOG2 Expression. PLoS One 10: e0135867.
21.
Markowitz J, Wesolowski R, Papenfuss T, et al. (2013): Myeloid-derived suppressor cells in breast cancer. Breast Cancer Res Treat 140: 13-21.
22.
Ostanin DV, Bhattacharya D (2013): Myeloid-derived suppressor cells in the inflammatory bowel diseases. Inflamm Bowel Dis 19: 2468-2477.
23.
Tang F, Wang F, An L, et al. (2015): Upregulation of Tim-3 on CD4(+) T cells is associated with Th1/Th2 imbalance in patients with allergic asthma. Int J Clin Exp Med 8: 3809-3816.
24.
Luo Y, Deng Y, Tao Z, et al. (2014): Regulatory effect of microRNA-135a on the Th1/Th2 imbalance in a murine model of allergic rhinitis. Exp Ther Med 8: 1105-1110.
25.
Lu TX, Hartner J, Lim EJ, et al. (2011): MicroRNA-21 limits in vivo immune response-mediated activation of the IL-12/IFN-gamma pathway, Th1polarization, and the severity of delayed-type hypersensitivity. J Immunol 187: 3362-3373.
26.
Möhnle P, Schütz SV, van der Heide V, et al. (2015): MicroRNA-146a controls Th1-cell differentiation of human CD4+ T lymphocytes by targeting PRKC. Eur J Immunol 45: 260-272.
27.
Mattes J, Collison A, Plank M, et al. (2009): Antagonism of microRNA-126 suppresses the effector function of TH2 cells and the development of allergic airways disease. Proc Natl Acad Sci U S A 106: 18704-18709.
28.
Kai W, Qian XU, Qun WU (2015): MicroRNAs and Asthma Regulation. Iran J Allergy Asthma Immunol 14: 120-125.
29.
Saito Y, Saito H (2012): Role of CTCF in the regulation of microRNA expression. Front Genet 3: 186.
30.
Chavali PL, Funa K, Chavali S, et al. (2011): Cis-regulation of microRNA expression by scaffold/matrix-attachment regions. Nucleic Acids Res 39: 6908-6918.
31.
Zhang X, Zhang B, Gao J, et al. (2013): Regulation of the microRNA 200b (miRNA-200b) by transcriptional regulators PEA3 and ELK-1 protein affectsexpression of Pin1 protein to control anoikis. J Biol Chem 288: 32742-32752.
Share
RELATED ARTICLE
| eISSN: | 1644-4124 |
| ISSN: | 1426-3912 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
