Basic fibroblast growth factor (bFGF) is one of the key and most extensively investigated angiogenic cytokine. Its activity has been evaluated in different in vitro as well as in vivo experimental models. We have previously shown, that human serum but also human bFGF induced significant neovascular response in the mice cutaneous assay. The aim of the present study was to confirm the direct proangiogenic effect of human bFGF in the mice in vivo model. Therefore, the effect of human serum absorption with anti-bFGF antibody on its angiogenic activity in serum-induced mice angiogenesis test (SIA) has been evaluated. As a result, significantly diminished angiogenic activity of sera preincubated with the anti-bFGF antibody has been demonstrated, with negligible effect of the normal goat IgG serving as a control. Consequently, the significant direct role of human bFGF in the neovascularization induced by human serum in the mice skin has been proven. Present study clearly demonstrated as well that mice cutaneous assay might serve as the in vivo model for monitoring of the angiogenic effect of human bFGF.