Mucosal chemokines are considered to be involved in the pathogenesis of Helicobacter pylori associated gastritis as well as gastric adenocarcinoma as a consequence of chronic inflammation process. Interleukin 8 (IL-8) produced by infected epithelial cells plays an important role in active inflammation by the induction of neutrophil migration and activation. The aim of a study was to investigate the effect of ethanol extract of propolis from Poland (EEPP) on IL-8 release by H. pylori infected human gastric adenocarcinoma cells (AGS).
Materials and methods: The AGS line cells was incubated with bacteria (bacteria to cell ratio was 100 : 1) in the absence (control) or the presence of tested solutions of EEPP (7.5-60 g/ml) for 16 hours. The concentrations of IL-8 were determined in culture supernatants by ELISA method.
Results: Incubation of AGS cells in presence of EEPP significantly suppressed IL-8 release in comparison with the control (p < 0.01). Obtained data has shown that IL-8 inhibition of EEPP was dose dependent. Additionally, 90 g/ml of EEPP significantly inhibited growth of H. pylori (p < 0.05). These results indicate that EEPP preparations might partially inhibit H. pylori-induced gastric inflammation by decreasing IL-8 production.