Central European Journal of Immunology
eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Share:
Share:
Original paper

Neutrophil elastase promotes mucin secretion in airway epithelial cells through the MARCKS/ACK1/cortactin pathway

Mingxin He
1
,
Qi Li
2
,
SHUYUAN OUYANG
1
,
Yan Liu
3
,
Juliy M. Perelman
4
,
Victor P. Kolosov
4
,
Xiangdong Zhou
2
,
Yanyan Li
1

  1. Department of Intensive Care Unit, Peking University Shenzhen Hospital, China
  2. Department of Respiratory Medicine, The First Affiliated Hospital, Hainan Medical University, China
  3. Department of Neurology, Shenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, China
  4. Far Eastern Scientific Center of Physiology and Pathology of Respiration, Blagoveshchensk, Russian Federation
Cent Eur J Immunol 2025; 50 (3)
Online publish date: 2025/10/13
Article files
- Neutrophil elastase.pdf  [4.17 MB]
Get citation
 
PlumX metrics:
 
1. Rogers DF (2007): Physiology of airway mucus secretion and pathophysiology of hypersecretion. Respir Care 52: 1134-1146.
2. Saco TV, Breitzig MT, Lockey RF, et al. (2018): Epigenetics of mucus hypersecretion in chronic respiratory diseases. Am J Respir Cell Mol Biol 58: 299-309.
3. Jaramillo AM, Vladar EK, Holguin F, et al. (2024): Emerging cell and molecular targets for treating mucus hypersecretion in asthma. Allergol Int 73: 375-381.
4. Hill DB, Button B, Rubinstein M, et al. (2022): Physiology and pathophysiology of human airway mucus. Physiol Rev 102: 1757-1836.
5. Svitkina TM (2020): Actin cell cortex: structure and molecular organization. Trends Cell Biol 30: 556-565.
6. Chugh P, Paluch EK (2018): The actin cortex at a glance. J Cell Sci 131: 186254.
7. Bandela M, Belvitch P, Garcia JGN, et al. (2022): Cortactin in lung cell function and disease. Int J Mol Sci 23: 4606.
8. Belvitch P, Htwe YM, Brown ME, et al. (2018): Cortical actin dynamics in endothelial permeability. Curr Top Membr 82: 141-195.
9. Ponce AG, Madrid AFC, Robles HV, et al. (2016): Loss of cortactin causes endothelial barrier dysfunction via disturbed adrenomedullin secretion and actomyosin contractility. Sci Rep 6: 29003.
10. Liu C, Li Q, Zhou X, et al. (2013): Cortactin mediates elevated shear stress-induced mucin hypersecretion via actin polymerization in human airway epithelial cells. Int J Biochem Cell Biol 45: 2756-2763.
11. Voynow JA, Shinbashi M (2021): Neutrophil elastase and chronic lung disease. Biomolecules 11: 1065.
12. Gornowicz-Porowska J, Bowszyc-Dmochowska M, Seraszek-Jaros A, et al. (2014): Cutaneous expressions of interleukin-6 and neutrophil elastase as well as levels of serum IgA antibodies to gliadin nonapeptides, tissue transglutaminase and epidermal transglutaminase: implications for both autoimmunity and autoinflammation involvement in dermatitis herpetiformis. Cent Eur J Immunol 39: 331-337.
13. Flores CFY, de Las Mercedes Hurtado Pineda Á, Bonilla VMC, et al. (2020): Sample management: stability of plasma and serum on different storage conditions. Ejifcc 31: 46-55.
14. Sheats MK, Yin Q, Fang S, et al. (2019): Marcks and lung disease. Am J Respir Cell Mol Biol 60: 16-27.
15. Eckert RE, Neuder LE, Park J, et al. (2010): Myristoylated alanine-rich c-kinase substrate (marcks) protein regulation of human neutrophil migration. Am J Respir Cell Mol Biol 42: 586-594.
16. Estrada-Bernal A, Gatlin JC, Sunpaweravong S, et al. (2009): Dynamic adhesions and marcks in melanoma cells. J Cell Sci 122: 2300-2310.
17. Salli U, Saito N, Stormshak F (2003): Spatiotemporal interactions of myristoylated alanine-rich c kinase substrate (marcks) protein with the actin cytoskeleton and exocytosis of oxytocin upon prostaglandin f2alpha stimulation of bovine luteal cells. Biol Reprod 69: 2053-2058.
18. Chen P, Deng Z, Wang T, et al. (2013): The potential interaction of marcks-related peptide and diltiazem on acrolein-induced airway mucus hypersecretion in rats. Int Immunopharmacol 17: 625-632.
19. Agrawal A, Rengarajan S, Adler KB, et al. (2007): Inhibition of mucin secretion with marcks-related peptide improves airway obstruction in a mouse model of asthma. J Appl Physiol (1985) 102: 399-405.
20. Modzelewska K, Newman LP, Desai R, et al. (2006): Ack1 mediates cdc42-dependent cell migration and signaling to p130cas. J Biol Chem 281: 37527-37535.
21. Lin Q, Lo CG, Cerione RA, et al. (2002): The cdc42 target ack2 interacts with sorting nexin 9 (sh3px1) to regulate epidermal growth factor receptor degradation. J Biol Chem 277: 10134-10138.
22. He L, Lin Y, Ge Z, et al. (2019): The legionella pneumophila effector wipa disrupts host f-actin polymerisation by hijacking phosphotyrosine signalling. Cell Microbiol 21: e13014.
23. Lažetić V, Joseph BB, Bernazzani SM, et al. (2018): Actin organization and endocytic trafficking are controlled by a network linking nima-related kinases to the cdc-42-sid-3/ack1 pathway. PLoS Genet 14: e1007313.
24. Posch W, Lass-Flörl C, Wilflingseder D (2021): SARS-cov-2-infected primary human airway epithelia illustrate mucus hypersecretion. J Allergy Clin Immunol 148: 909.
25. Prasher P, Sharma M (2022): Targeting mucin hypersecretion in covid-19 therapy. Future Med Chem 14: 681-684.
26. Singanayagam A, Footitt J, Marczynski M, et al. (2022): Airway mucins promote immunopathology in virus-exacerbated chronic obstructive pulmonary disease. J Clin Invest 132: e120901.
27. Jaramillo AM, Azzegagh Z, Tuvim MJ, et al. (2018): Airway mucin secretion. Ann Am Thorac Soc 15: S164-S170.
28. Adler KB, Tuvim MJ, Dickey BF (2013): Regulated mucin secretion from airway epithelial cells. Front Endocrinol (Lausanne) 4: 129.
29. Jin BY, Lin AJ, Golan DE, et al. (2012): Marcks protein mediates hydrogen peroxide regulation of endothelial permeability. Proc Natl Acad Sci U S A 109: 14864-14869.
30. Lin K, Fang S, Park J, et al. (2010): Marcks and related chaperones bind to unconventional myosin v isoforms in airway epithelial cells. Am J Respir Cell Mol Biol 43: 131-136.
31. Polosukhin VV, Cates JM, Lawson WE, et al. (2011): Hypoxia-inducible factor-1 signalling promotes goblet cell hyperplasia in airway epithelium. J Pathol 224: 203-211.
32. Green TD, Crews AL, Park J, et al. (2011): Regulation of mucin secretion and inflammation in asthma: a role for marcks protein. Biochim Biophys Acta 1810: 1110-1113.
33. Park J, Crews AL, Lampe WR, et al. (2007): Protein kinase c delta regulates airway mucin secretion via phosphorylation of marcks protein. Am J Pathol 171: 1822-1830.
34. Jeannot P, Besson A (2020): Cortactin function in invadopodia. Small Gtpases 11: 256-270.
35. González-Jamett AM, Guerra MJ, Olivares MJ, et al. (2017): The f-actin binding protein cortactin regulates the dynamics of the exocytotic fusion pore through its sh3 domain. Front Cell Neurosci 11: 130.
36. Schnoor M, Stradal TE, Rottner K (2018): Cortactin: cell functions of a multifaceted actin-binding protein. Trends Cell Biol 28: 79-98.
37. Wang R, Cleary RA, Wang T, et al. (2014): The association of cortactin with profilin-1 is critical for smooth muscle contraction. J Biol Chem 289: 14157-14169.
38. Brescia G, Parrino D, Nicolè L, et al. (2018): Cortactin expression in nasal polyps of aspirin-exacerbated respiratory disease (AERD) patients. Am J Otolaryngol 39: 293-298.
39. Cleary RA, Wang R, Waqar O, et al. (2014): Role of c-abl tyrosine kinase in smooth muscle cell migration. Am J Physiol Cell Physiol 306: C753-C761.
40. Rizzo AN, Aman J, van Nieuw Amerongen GP, et al. (2015): Targeting abl kinases to regulate vascular leak during sepsis and acute respiratory distress syndrome. Arterioscler Thromb Vasc Biol 35: 1071-1079.
41. Yu D, Makkar G, Strickland DK, et al. (2015): Myristoylated alanine-rich protein kinase substrate (MARCKS) regulates small GTPase rac1 and cdc42 activity and is a critical mediator of vascular smooth muscle cell migration in intimal hyperplasia formation. J Am Heart Assoc 4: e002255.
42. Mahajan K, Mahajan NP (2015): Ack1/tnk2 tyrosine kinase: molecular signaling and evolving role in cancers. Oncogene 34: 4162-4167.
43. Jing L, Zhang X, Liu D, et al. (2022): ACK1 contributes to the pathogenesis of inflammation and autoimmunity by promoting the activation of TLR signaling pathways. Front Immunol 13: 864995.
Copyright: © 2025 Polish Society of Experimental and Clinical Immunology This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
Quick links
© 2025 Termedia Sp. z o.o.
Developed by Bentus.