Central European Journal of Immunology
eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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abstract:
Original paper

LAMA1 derived from colorectal cancer promotes M2 polarization in macrophages via activation of the EGFR/AKT/CREB pathway

Jiying Lu
1
,
Chenyang Ge
1
,
Pengcheng Yu
1
,
Zhekang Jin
1
,
Mengxiang Yang
1
,
Xihan Jin
1

  1. Department of Colorectal and Anal Surgery, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, China
(Cent Eur J Immunol 2025; 50 (3)
DOI: https://doi.org/10.5114/ceji.2025.154516
Online publish date: 2025/11/06
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Introduction
Although the development of certain malignancies is linked to aberrant expression of LAMA1, it is not yet known how precisely LAMA1 regulates macrophage M2 polarization and the malignant evolution of colorectal cancer (CRC).

Material and methods
qRT-PCR and western blot (WB) were used to identify the expression of LAMA1 in human normal colonic epithelial cells (NCM-460) and CRC cell lines (RKO, LoVo). Using ELISA kits, the protein levels of LAMA1, interleukin (IL)-10, and Arg1 were determined. The assays used in the study included flow cytometry to evaluate CRC cell apoptosis and macrophage M2 polarization. Colony formation assessed the proliferative ability of co-cultured CRC cells, with Transwell assessing migration and invasion. WB identified the expression of proteins linked to the epithelial- mesenchymal transition (EMT).

Results
In CRC cells, LAMA1 was overexpressed. LAMA1 generated from CRC stimulated the EGFR/AKT/CREB signaling pathway in macrophages to induce M2 polarization of macrophages and eventually promote CRC cell proliferation, migration, and invasion, as well as to activate the EMT process and block CRC cell apoptosis.

Conclusions
As a pro-carcinogenic factor released by CRC cells, LAMA1 affects the activation of the EGFR/AKT/CREB pathway in macrophages, causing M2 polarization and aggravating the malignant evolution of CRC.

keywords:

colorectal cancer, EGFR, LAMA1, macrophages, M2 polarization
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